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Upadhyay And Upadhyay Biophysical Chemistry Pdf 29



 


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. This is based on the electronic charge on the carboxylate group of each ion.. Why the limit of pH 5.0? By the same reason. a. If the reaction mixture contains an ion of the same charge as the salt of the buffer, the ion will dissolve. b. However, a strong acid or base ion will not dissolve and hence, the pH of the solution will be 5.0. c. The reasons for the dissolving property is based on the Van't Hoff Law. pKa of a salt like NaCl is equal to the logarithm of the mole concentration of the salt raised to the power of the pKa of the salt. pKa of KCl = 3.6. pKa of NaCl = 1.0 pKa of pH 4.0- 5.0 buffer. Hence, for aqueous salt solutions, the logarithm of the mole concentration raised to the power of 1.0 should equal the pH. How will the pH of the buffer change? A) If the mol concentration of the salt increases. the pKa increases. b) If the mol concentration of the salt decreases. the pKa decreases.. pKa is 1.0 (HCl). pKa of HNO3 = -12.6. pKa of H2SO4 = -4.6. pKa of KCl = 3.6 pKa of NaCl = 1.0 pKa of pH 4.0- 5.0 buffer. Hence, for aqueous salt solutions, the logarithm of the mole concentration raised to the power of 1.0 should equal the pH. ABSTRACT: HIGH pH IS AN ENEMY TO BIOMASS PRODUCTION - An Alkaline environment will inhibit the activity of most enzymes. During biological synthesis, enzyme activity requires an optimum pH. This optimum pH is commonly referred to as the "optimum pH". The optimum pH is the pH at which maximal activity is achieved. "Optimum" is commonly used as a short form of "optimum pH" while "pH" is commonly used as a short form of "pH of activity". The "optimum pH" is widely defined as the pH at which the reaction is most efficient. Most experiments are conducted at different pH values, one at the "optimum pH" and one at "non-optimum pH". The "non-optimum pH" is usually set as the

 

 


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Upadhyay And Upadhyay Biophysical Chemistry Pdf 29

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